This study provides a method for determining various animal derived meats based on liquid chromatography tandem mass spectrometry technology, including sample pretreatment, screening of characteristic peptides in various standard meat samples, and detection of characteristic peptides in the tested meat samples using high-performance liquid chromatography-mass spectrometry. This method can be used to identify various animal derived meat components. The technology fully utilizes the powerful scanning function of liquid chromatography-mass spectrometry. By monitoring and selecting the ion monitoring mode to collect signals in real time, and triggering the secondary ion intensity, the corresponding mother ion secondary fragmentation full scan spectrum can be collected to obtain more accurate qualitative information, avoiding interference from complex matrices and reducing the probability of false positives. In addition, this method has simple preprocessing and is convenient for batch sample operation. The Research Team for Authenticity Identification of Animal Derived Foods (referred to as the "Team") is one of the core teams of the China Meat Products Comprehensive Research Center (National Meat Products Quality Supervision and Inspection Center). The team is mainly engaged in the quality and safety assurance of animal derived foods and the research and development of technical methods. The project team consists of 5 personnel, with reasonable professional, professional, and age combinations, and has a good cooperation foundation in the past. LC-MS/MS method was established to determine 5 animal proteins (pig, cattle, sheep, chicken, duck) and 3 plant proteins (pea, peanut, soybean). Published 1 EI paper: Identification of Duck Meat Adulteration in Lamb Meat by Liquid Chromatography Tandem Mass Spectrometry, Food Science, 2016, 37 (6): 204-209; Published 1 core journal paper: Identification and Quantitative Study of Adulterated Beef by Liquid Chromatography Tandem Mass Spectrometry, Modern Food Technology, 2017, 33 (2). Applied for two patents: ① Method for determining various animal derived meats based on liquid chromatography tandem mass spectrometry technology (201510937689.5); ② Method for determination of multiple plant derived components in meat products based on liquid chromatography tandem mass spectrometry technology (201710161919.2)